Output below are interactive Manhattan plots. The x-axis has functional terms and the y-axis has the -log10(adjust p-value). The size of the circles corresponds to the term size. Term size is the number of genes in the pathway. So, GO:1234 (5) would have a smaller circle compared to GO:5678 (100) because there are 5 genes in GO:1234 pathway vs 100 genes in the GO:5678 pathway. These plots are only showing significant results. This is why some plots cannot be generated despite 10 genes used for input into the analysis. We will use genes with an adjusted p-value < 0.05 and only usin data sources from GO:MF, GO:BP, KEGG, and TF.

Age

Pericytes & SMCs

Up-regulated

runGprofiler("age","Pericytes & SMCs","up-regulated")
## [1] "83 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Pericytes & SMCs","down-regulated")
## [1] "21 genes input into functional enrichment analysis."

Vwf+Cldn5+ BECs

Up-regulated

runGprofiler("age","Vwf+Cldn5+ BECs","up-regulated")
## [1] "17 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Vwf+Cldn5+ BECs","down-regulated")
## [1] "13 genes input into functional enrichment analysis."

Vwf+ BECs & LECs

Up-regulated

runGprofiler("age","Vwf+ BECs & LECs","up-regulated")
## [1] "50 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Vwf+ BECs & LECs","down-regulated")
## [1] "75 genes input into functional enrichment analysis."

Kdr-high BECs & LECs

Up-regulated

runGprofiler("age","Kdr-high BECs & LECs","up-regulated")
## [1] "86 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Kdr-high BECs & LECs","down-regulated")
## [1] "72 genes input into functional enrichment analysis."

Doublets BECs / macrophages

Up-regulated

runGprofiler("age","Doublets BECs / macrophages","up-regulated")
## [1] "3 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Doublets BECs / macrophages","down-regulated")
## [1] "2 genes input into functional enrichment analysis."

Mrc1+H2-Eb1-low macrophages

Up-regulated

runGprofiler("age","Mrc1+H2-Eb1-low macrophages","up-regulated")
## [1] "215 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Mrc1+H2-Eb1-low macrophages","down-regulated")
## [1] "159 genes input into functional enrichment analysis."

Mrc1+H2-Eb1-high macrophages

Up-regulated

runGprofiler("age","Mrc1+H2-Eb1-high macrophages","up-regulated")
## [1] "84 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Mrc1+H2-Eb1-high macrophages","down-regulated")
## [1] "37 genes input into functional enrichment analysis."

Dendritic cells

Up-regulated

runGprofiler("age","Dendritic cells","up-regulated")
## [1] "387 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Dendritic cells","down-regulated")
## [1] "70 genes input into functional enrichment analysis."

Monocytes

Up-regulated

runGprofiler("age","Monocytes","up-regulated")
## [1] "18 genes input into functional enrichment analysis."

Down-regulated

# 3 genes input, no results to show

Neutrophils

Up-regulated

runGprofiler("age","Neutrophils","up-regulated")
## [1] "118 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Neutrophils","down-regulated")
## [1] "26 genes input into functional enrichment analysis."

Mast cells

Up-regulated

runGprofiler("age","Mast cells","up-regulated")
## [1] "59 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Mast cells","down-regulated")
## [1] "20 genes input into functional enrichment analysis."

Myeloid progenitors

Up-regulated

runGprofiler("age","Myeloid progenitors","up-regulated")
## [1] "24 genes input into functional enrichment analysis."

Down-regulated

# 1 gene input, no results to show

Fibroblasts

Up-regulated

runGprofiler("age","Fibroblasts","up-regulated")
## [1] "114 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Fibroblasts","down-regulated")
## [1] "82 genes input into functional enrichment analysis."

Schwann cells

Up-regulated

runGprofiler("age","Schwann cells","up-regulated")
## [1] "117 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Schwann cells","down-regulated")
## [1] "16 genes input into functional enrichment analysis."

T cells & ILCs

Up-regulated

runGprofiler("age","T cells & ILCs","up-regulated")
## [1] "100 genes input into functional enrichment analysis."

Down-regulated

# 3 genes used for analysis
# only has significant results in the MIRNA source

Developing B cells

Up-regulated

runGprofiler("age","Developing B cells","up-regulated")
## [1] "257 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Developing B cells","down-regulated")
## [1] "25 genes input into functional enrichment analysis."

Mature B cells

Up-regulated

runGprofiler("age","Mature B cells","up-regulated")
## [1] "261 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("age","Mature B cells","down-regulated")
## [1] "100 genes input into functional enrichment analysis."

Isoform

Pericytes & SMCs

Up-regulated

# 4 genes input, no results to show

Down-regulated

# 0 genes input, no results to show

Vwf+Cldn5+ BECs

Up-regulated

# 1 gene input, no results to show

Down-regulated

runGprofiler("isoform","Vwf+Cldn5+ BECs","down-regulated")
## [1] "17 genes input into functional enrichment analysis."

Vwf+ BECs & LECs

Up-regulated

runGprofiler("isoform","Vwf+ BECs & LECs","up-regulated")
## [1] "28 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("isoform","Vwf+ BECs & LECs","down-regulated")
## [1] "15 genes input into functional enrichment analysis."

Kdr-high BECs & LECs

Up-regulated

runGprofiler("isoform","Kdr-high BECs & LECs","up-regulated")
## [1] "17 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("isoform","Kdr-high BECs & LECs","down-regulated")
## [1] "22 genes input into functional enrichment analysis."

Doublets BECs / macrophages

Up-regulated

# 0 genes input, no results to show

Down-regulated

# 1 gene input, no results to show

Mrc1+H2-Eb1-low macrophages

Up-regulated

runGprofiler("isoform","Mrc1+H2-Eb1-low macrophages","up-regulated")
## [1] "202 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("isoform","Mrc1+H2-Eb1-low macrophages","down-regulated")
## [1] "82 genes input into functional enrichment analysis."

Mrc1+H2-Eb1-high macrophages

Up-regulated

runGprofiler("isoform","Mrc1+H2-Eb1-high macrophages","up-regulated")
## [1] "34 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("isoform","Mrc1+H2-Eb1-high macrophages","down-regulated")
## [1] "18 genes input into functional enrichment analysis."

Dendritic cells

Up-regulated

runGprofiler("isoform","Dendritic cells","up-regulated")
## [1] "28 genes input into functional enrichment analysis."

Down-regulated

# 9 genes input, no results to show

Monocytes

Up-regulated

runGprofiler("isoform","Monocytes","up-regulated")
## [1] "3 genes input into functional enrichment analysis."

Down-regulated

# 3 genes input, no results to show

Neutrophils

Up-regulated

runGprofiler("isoform","Neutrophils","up-regulated")
## [1] "3 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("isoform","Neutrophils","down-regulated")
## [1] "6 genes input into functional enrichment analysis."

Mast cells

Up-regulated

# 3 genes input, no results to show

Down-regulated

# no genes input, no results to show

Myeloid progenitors

Up-regulated

runGprofiler("isoform","Myeloid progenitors","up-regulated")
## [1] "45 genes input into functional enrichment analysis."

Down-regulated

# 2 genes input, no results to show

Fibroblasts

Up-regulated

runGprofiler("isoform","Fibroblasts","up-regulated")
## [1] "14 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("isoform","Fibroblasts","down-regulated")
## [1] "24 genes input into functional enrichment analysis."

Schwann cells

Up-regulated

runGprofiler("isoform","Schwann cells","up-regulated")
## [1] "104 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("isoform","Schwann cells","down-regulated")
## [1] "26 genes input into functional enrichment analysis."

T cells & ILCs

Up-regulated

runGprofiler("isoform","T cells & ILCs","up-regulated")
## [1] "32 genes input into functional enrichment analysis."

Down-regulated

# 3 genes used for analysis
# only has significant results in the MIRNA source

Developing B cells

Up-regulated

runGprofiler("isoform","Developing B cells","up-regulated")
## [1] "31 genes input into functional enrichment analysis."

Down-regulated

# 4 genes input, no results to show

Mature B cells

Up-regulated

runGprofiler("isoform","Mature B cells","up-regulated")
## [1] "96 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("isoform","Mature B cells","down-regulated")
## [1] "9 genes input into functional enrichment analysis."

Sex

Pericytes & SMCs

Up-regulated

# 3 genes input, no results to show

Down-regulated

runGprofiler("sex","Pericytes & SMCs","down-regulated")
## [1] "7 genes input into functional enrichment analysis."

Vwf+Cldn5+ BECs

Up-regulated

runGprofiler("sex","Vwf+Cldn5+ BECs","up-regulated")
## [1] "18 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("sex","Vwf+Cldn5+ BECs","down-regulated")
## [1] "7 genes input into functional enrichment analysis."

Vwf+ BECs & LECs

Up-regulated

runGprofiler("sex","Vwf+ BECs & LECs","up-regulated")
## [1] "51 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("sex","Vwf+ BECs & LECs","down-regulated")
## [1] "19 genes input into functional enrichment analysis."

Kdr-high BECs & LECs

Up-regulated

runGprofiler("sex","Kdr-high BECs & LECs","up-regulated")
## [1] "57 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("sex","Kdr-high BECs & LECs","down-regulated")
## [1] "34 genes input into functional enrichment analysis."

Doublets BECs / macrophages

Up-regulated

# 2 genes input, no results to show

Down-regulated

runGprofiler("sex","Doublets BECs / macrophages","down-regulated")
## [1] "4 genes input into functional enrichment analysis."

Mrc1+H2-Eb1-low macrophages

Up-regulated

runGprofiler("sex","Mrc1+H2-Eb1-low macrophages","up-regulated")
## [1] "46 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("sex","Mrc1+H2-Eb1-low macrophages","down-regulated")
## [1] "61 genes input into functional enrichment analysis."

Mrc1+H2-Eb1-high macrophages

Up-regulated

runGprofiler("sex","Mrc1+H2-Eb1-high macrophages","up-regulated")
## [1] "23 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("sex","Mrc1+H2-Eb1-high macrophages","down-regulated")
## [1] "31 genes input into functional enrichment analysis."

Dendritic cells

Up-regulated

# 9 genes input, no results to show

Down-regulated

runGprofiler("sex","Dendritic cells","down-regulated")
## [1] "34 genes input into functional enrichment analysis."

Monocytes

Up-regulated

# 6 genes input, no results to show

Down-regulated

runGprofiler("sex","Monocytes","down-regulated")
## [1] "14 genes input into functional enrichment analysis."

Neutrophils

Up-regulated

runGprofiler("sex","Neutrophils","up-regulated")
## [1] "28 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("sex","Neutrophils","down-regulated")
## [1] "39 genes input into functional enrichment analysis."

Mast cells

Up-regulated

runGprofiler("sex","Mast cells","up-regulated")
## [1] "18 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("sex","Mast cells","down-regulated")
## [1] "36 genes input into functional enrichment analysis."

Myeloid progenitors

Up-regulated

# 2 genes input, no results to show

Down-regulated

runGprofiler("sex","Myeloid progenitors","down-regulated")
## [1] "4 genes input into functional enrichment analysis."

Fibroblasts

Up-regulated

runGprofiler("sex","Fibroblasts","up-regulated")
## [1] "67 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("sex","Fibroblasts","down-regulated")
## [1] "23 genes input into functional enrichment analysis."

Schwann cells

Up-regulated

runGprofiler("sex","Schwann cells","up-regulated")
## [1] "71 genes input into functional enrichment analysis."

Down-regulated

runGprofiler("sex","Schwann cells","down-regulated")
## [1] "33 genes input into functional enrichment analysis."

T cells & ILCs

Up-regulated

runGprofiler("sex","T cells & ILCs","up-regulated")
## [1] "11 genes input into functional enrichment analysis."

Down-regulated

# 3 genes used for analysis
# only has significant results in the MIRNA source

Developing B cells

Up-regulated

# 3 genes input, no results to show

Down-regulated

# 8 genes input, no results to show

Mature B cells

Up-regulated

# 5 genes input, no results to show

Down-regulated

runGprofiler("sex","Mature B cells","down-regulated")
## [1] "107 genes input into functional enrichment analysis."